Just south of Charles Square is Charles University’s botanical garden. It was founded in 1775 and actually moved from Smíchov district to its present site in 1898. It is the Czechs’ oldest botanical garden. The steep, hillside garden concentrates on Central European flora and is especially pretty in spring.
Location: Viničná 7 (entrances on Viničná and Vyšehradská); admission free; open 9am-6pm
The gardens have specimens from around the world including a huge succulent display, hardly Central Europe. It is an extremely pleasant garden just to stroll through but also almost every plant is labelled with much information in English.